Production, Optimization, Purification and Characterization of Mannanase Produced by Bacillus Subtilis
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چکیده
منابع مشابه
Isolation, Purification and Characterization of Mannanase from Bacillus subtilis MAN-511
A bacterium, MAN-511 that produced extracellular mannanase, was isolated and identified as Bacillus subtilis on the basis of 16S rDNA phylogenetic analysis. The enzyme was purified to apparent homogeneity by precipitasi ammonium sulfat 70% and Sephadex G-75 chromatography procedures. The mannanase was purified 7.6 fold and specificity of 9.3 U/mg protein. SDS-PAGE of the purified enzyme showed ...
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Cloning and expression of the L-phenylalanine dehydrogenase gene, from B. sphaericus in E. coli were done. The gene was cloned in the vector pET16b and transformed into E. coli BL21 (DE3). The functional form of the L-phenylalanine dehydrogenase enzyme was purified by affinity purification techniques, taking advantage of the ability of this enzyme to bind to the nucleotide site affinity dye, Re...
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ژورنال
عنوان ژورنال: Journal of Advances in Microbiology
سال: 2020
ISSN: 2456-7116
DOI: 10.9734/jamb/2020/v20i630254